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Western blot using GeneTexs Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5).? Approximately 10 microg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature.? Signal was detected using standard techniques.
Western blot using GeneTexs Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5).? Approximately 10 microg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature.? Signal was detected using standard techniques.
Western blot using GeneTexs Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5).? Approximately 10 microg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature.? Signal was detected using standard techniques.

PNK antibody

GTX24191
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Primate
TargetPNKP
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Overview

  • Supplier
    GeneTex
  • Product Name
    PNK antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:300-1:500. ELISA: 1:3000-1:12000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.46 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11284
  • Target name
    PNKP
  • Target description
    polynucleotide kinase 3'-phosphatase
  • Target synonyms
    AOA4; bifunctional polynucleotide phosphatase/kinase; CMT2B2; DNA 5'-kinase/3'-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); MCSZ; PNK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T60
  • Protein Name
    Bifunctional polynucleotide phosphatase/kinase
  • Scientific Description
    Mammalian PNK catalyzes the 5-prime phosphorylation of nucleic acids and can have associated 3-prime phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.
  • Reactivity
    Human, Primate
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203