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Western blot analysis of POLD3 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).
Western blot analysis of POLD3 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).
Western blot analysis of POLD3 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the POLD3 antibody detected the POLD3 protein (arrow).

POLD3 (C-term) Rabbit Polyclonal Antibody

AP53378PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetPOLD3
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    POLD3 (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10714
  • Target name
    POLD3
  • Target description
    DNA polymerase delta 3, accessory subunit
  • Target synonyms
    IMD122, P66, P68, PPP1R128, DNA polymerase delta subunit 3, DNA polymerase delta subunit C, DNA polymerase delta subunit p66, DNA polymerase delta subunit p68, Pol delta C subunit (p66), polymerase (DNA) delta 3, accessory subunit, polymerase (DNA-directed), delta 3, accessory subunit, protein phosphatase 1, regulatory subunit 128
  • Host
    Rabbit
  • Protein IDQ15054
  • Protein Name
    DNA polymerase delta subunit 3
  • Scientific Description
    POLD3 (C-term) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of POLD3 using anti-POLD3 antibody (A05111-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLD3 antigen affinity purified polyclonal antibody (Catalog # A05111-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLD3 at approximately 70 kDa. The expected band size for POLD3 is at 51 kDa.
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