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POLDIP2 Antibody, Biotin conjugated

CSB-PA896496LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPOLDIP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLDIP2 Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID26073
  • Target name
    POLDIP2
  • Target description
    DNA polymerase delta interacting protein 2
  • Target synonyms
    PDIP38, p38, polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, polymerase (DNA) delta interacting protein 2, polymerase (DNA-directed), delta interacting protein 2, polymerase delta interacting protein 38
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2S7
  • Protein Name
    Polymerase delta-interacting protein 2
  • Scientific Description
    mitochondrial nucleoid, mitochondrion
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLDIP2 using anti-POLDIP2 antibody (A09581-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLDIP2 antigen affinity purified polyclonal antibody (Catalog # A09581-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLDIP2 at approximately 36 kDa. The expected band size for POLDIP2 is at 42 kDa.
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