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Western Blot analysis of HeLa cells using DNA pol iota Polyclonal Antibody
Western Blot analysis of HeLa cells using DNA pol iota Polyclonal Antibody
Western Blot analysis of HeLa cells using DNA pol iota Polyclonal Antibody

POLI Antibody

CSB-PA006790
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPOLI
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLI Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11201
  • Target name
    POLI
  • Target description
    DNA polymerase iota
  • Target synonyms
    DNA polymerase iota; eta2; polymerase (DNA directed) iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD30 homolog B; RAD30B; RAD3OB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNA4
  • Protein Name
    DNA polymerase iota
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-DNA Polymerase iota Polyclonal Antibody, Unconjugated (bs-13018R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
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Figure 1. Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody (Catalog # A01376-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.
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