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ProductsBack/DNA polymerase iota antibody
The data was published in the 2021 in J Biol Chem.
The data was published in the 2021 in J Biol Chem.
The data was published in the 2021 in J Biol Chem.

DNA polymerase iota antibody

GTX112137
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPOLI
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Overview

  • Supplier
    GeneTex
  • Product Name
    DNA polymerase iota antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11201
  • Target name
    POLI
  • Target description
    DNA polymerase iota
  • Target synonyms
    RAD30B, RAD3OB, eta2, DNA polymerase iota, RAD30 homolog B, polymerase (DNA directed) iota, polymerase (DNA) iota, polymerase (DNA-directed), iota
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNA4
  • Protein Name
    DNA polymerase iota
  • Scientific Description
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hutcherson RJ, Gabbard RD, Castellanos AJ, et al. Age and insulin-like growth factor-1 impact PCNA monoubiquitination in UVB-irradiated human skin. J Biol Chem. 2021,296:100570. doi: 10.1016/j.jbc.2021.100570
    Read this paper
  • Deng X, Yan Z, Cheng F, et al. Replication of an Autonomous Human Parvovirus in Non-dividing Human Airway Epithelium Is Facilitated through the DNA Damage and Repair Pathways. PLoS Pathog. 2016,12(1):e1005399. doi: 10.1371/journal.ppat.1005399
    Read this paper

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Figure 1. Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody (Catalog # A01376-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.
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