
WB analysis of human B lymphoblast cells using GTX46189 POLK antibody at 0.2-1μg/ml.
POLK antibody, Internal
GTX46189
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPOLK
Overview
- SupplierGeneTex
- Product NamePOLK antibody, Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- ConjugateUnconjugated
- Gene ID51426
- Target namePOLK
- Target descriptionDNA polymerase kappa
- Target synonymsDINB1, DINP, POLQ, DNA polymerase kappa, polymerase (DNA directed) kappa
- HostRabbit
- IsotypeIgG
- Protein IDQ9UBT6
- Protein NameDNA polymerase kappa
- Scientific DescriptionThis gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161





![Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4o followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48495/Polk-antibody-GTX48495-WB-1_w_23060823_120.webp)

