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Western Blot analysis of 293 cells using POLL Polyclonal Antibody
Western Blot analysis of 293 cells using POLL Polyclonal Antibody
Western Blot analysis of 293 cells using POLL Polyclonal Antibody

POLL Antibody

CSB-PA002174
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOLL
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Overview

  • Supplier
    Cusabio
  • Product Name
    POLL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27343
  • Target name
    POLL
  • Target description
    DNA polymerase lambda
  • Target synonyms
    BETAN, POLKAPPA, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, polymerase (DNA directed), lambda, polymerase (DNA) lambda
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UGP5
  • Protein Name
    DNA polymerase lambda
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNA Polymerase lambda using anti-DNA Polymerase lambda antibody (A00959-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase lambda antigen affinity purified polyclonal antibody (Catalog # A00959-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase lambda at approximately 70KD. The expected band size for DNA Polymerase lambda is at 63KD.
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