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ProductsBack/POLL / DNA Polymerase Lambda Antibody

POLL / DNA Polymerase Lambda Antibody

LS-C409943
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPOLL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POLL / DNA Polymerase Lambda Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 33kDa/63kDa, while the observed MW by Western blot was 68kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 33kDa/63kDa, while the observed MW by Western blot was 68kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27343
  • Target name
    POLL
  • Target description
    DNA polymerase lambda
  • Target synonyms
    BETAN, POLKAPPA, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, polymerase (DNA directed), lambda, polymerase (DNA) lambda
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNA Polymerase lambda using anti-DNA Polymerase lambda antibody (A00959-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase lambda antigen affinity purified polyclonal antibody (Catalog # A00959-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase lambda at approximately 70KD. The expected band size for DNA Polymerase lambda is at 63KD.
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