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POLR2B Antibody

CAB5928
Assay Genie
TargetPOLR2B
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    POLR2B Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5431
  • Target name
    POLR2B
  • Target description
    RNA polymerase II subunit B
  • Target synonyms
    POL2RB, RPB2, hRPB140, DNA-directed RNA polymerase II subunit RPB2, 3'-5' exoribonuclease, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II second largest subunit, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA-directed RNA polymerase II subunit RPB2, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, polymerase (RNA) II subunit B
  • Host
    Rabbit
  • Protein IDP30876
  • Protein Name
    DNA-directed RNA polymerase II subunit RPB2
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2B using anti-POLR2B antibody (A08699-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Ramos whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2B antigen affinity purified polyclonal antibody (Catalog # A08699-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2B at approximately 130 kDa. The expected band size for POLR2B is at 134 kDa.
Product group Antibodies
Boster Bio
Anti-POLR2B Antibody Picoband(r)A08699-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetPOLR2B
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Product group Antibodies
RayBiotech
Anti-POLR2B Antibody144-05928
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Product group Antibodies
Antibodies.com
Anti-POLR2B AntibodyA34914
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POLR2B AntibodyCSB-PA018328GA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
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Product group Antibodies
References
GeneTex
RPB2 antibody [N1], N-termGTX102535
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPOLR2B
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Product group Antibodies
LifeSpan BioSciences
POLR2B / RPB2 AntibodyLS-C334391
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPOLR2B
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-POLR2B AntibodyHPA037506
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
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TargetPOLR2B
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