
Sample (30 μg of whole cell lysate) A: BCL-1 5% SDS PAGE GTX102535 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RPB2 antibody [N1], N-term
GTX102535
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPOLR2B
Overview
- SupplierGeneTex
- Product NameRPB2 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.32 mg/ml
- ConjugateUnconjugated
- Gene ID5431
- Target namePOLR2B
- Target descriptionRNA polymerase II subunit B
- Target synonymsPOL2RB, RPB2, hRPB140, DNA-directed RNA polymerase II subunit RPB2, 3'-5' exoribonuclease, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II second largest subunit, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA-directed RNA polymerase II subunit RPB2, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, polymerase (RNA) II subunit B
- HostRabbit
- IsotypeIgG
- Protein IDP30876
- Protein NameDNA-directed RNA polymerase II subunit RPB2
- Scientific DescriptionThis gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161



![RPB2 antibody [N1], N-term detects RPB2 protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RPB2 antibody [N1], N-term (GTX102535) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min RPB2 antibody [N1], N-term detects RPB2 protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RPB2 antibody [N1], N-term (GTX102535) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102535/GTX102535_39918_20151227_IHC-P_w_23060100_818.webp)
![RPB2 antibody [N1], N-term detects RPB2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tongue. RPB2 stained by RPB2 antibody [N1], N-term (GTX102535) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min RPB2 antibody [N1], N-term detects RPB2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tongue. RPB2 stained by RPB2 antibody [N1], N-term (GTX102535) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102535/GTX102535_44006_20210326_IHC-P_M_w_23060100_190.webp)




![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1042] (GTX635976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635976/GTX635976_44340_20221209_WB_D_C_22121123_401.webp)
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221209_WB_D_C_22121123_648.webp)
