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POLR2D Polyclonal Antibody

RD82376A
Reddot Biotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPOLR2D
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    POLR2D Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5433
  • Target name
    POLR2D
  • Target description
    RNA polymerase II subunit D
  • Target synonyms
    DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; HSRBP4; HSRPB4; polymerase (RNA) II (DNA directed) polypeptide D; polymerase (RNA) II subunit D; RBP4; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RNA polymerase II subunit hsRPB4; RPB16; RPB4
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3 untranslated region of the transcript from this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2D using anti-POLR2D antibody (A12839-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (Catalog # A12839-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 16-20 kDa.
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