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Western blot analysis of POLR3GL Antibody (Center) in mouse kidney tissue lysates (35ug/lane). This demonstrates the POLR3GL antibody detected the POLR3GL protein (arrow).
Western blot analysis of POLR3GL Antibody (Center) in mouse kidney tissue lysates (35ug/lane). This demonstrates the POLR3GL antibody detected the POLR3GL protein (arrow).
Western blot analysis of POLR3GL Antibody (Center) in mouse kidney tissue lysates (35ug/lane). This demonstrates the POLR3GL antibody detected the POLR3GL protein (arrow).

POLR3GL (Center) Rabbit Polyclonal Antibody

AP53388PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPOLR3GL
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    POLR3GL (Center) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID84265
  • Target name
    POLR3GL
  • Target description
    RNA polymerase III subunit GL
  • Target synonyms
    RPC32HOM, SOFM, flj32422, DNA-directed RNA polymerase III subunit RPC7-like, DNA-directed RNA polymerase III subunit G-like, RNA polymerase III 32 kDa beta subunit, RNA polymerase III subunit C7-like, RNA polymerase III subunit G like, RPC32-beta, RPC32-like protein, alternative RNA polymerase III subunit 32, polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like, polymerase (RNA) III subunit G like
  • Host
    Rabbit
  • Protein IDQ9BT43
  • Protein Name
    DNA-directed RNA polymerase III subunit RPC7-like
  • Scientific Description
    POLR3GL (Center) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR3GL using anti-POLR3GL antibody (A17067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3GL antigen affinity purified polyclonal antibody (Catalog # A17067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3GL at approximately 24 kDa. The expected band size for POLR3GL is at 25 kDa.
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