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POMGNT2 / GTDC2 Antibody (aa351-400)

LS-C81337
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPOMGNT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POMGNT2 / GTDC2 Antibody (aa351-400)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84892
  • Target name
    POMGNT2
  • Target description
    protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-)
  • Target synonyms
    AGO61; C3orf39; glycosyltransferase-like domain containing 2; glycosyltransferase-like domain-containing protein 2; GTDC2; MDDGA8; MDDGC8; protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2; protein O-mannose beta-1,4-N-acetylglucosaminyltransferase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POMGNT2 using anti-POMGNT2 antibody (A11987-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMGNT2 antigen affinity purified polyclonal antibody (Catalog # A11987-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMGNT2 at approximately 75 kDa. The expected band size for POMGNT2 is at 67 kDa.
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