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Immunofluorescent analysis of HepG2 cells using CSB-PA613586LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA613586LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using CSB-PA613586LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PON2 Antibody

CSB-PA613586LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetPON2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PON2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5445
  • Target name
    PON2
  • Target description
    paraoxonase 2
  • Target synonyms
    serum paraoxonase/arylesterase 2, A-esterase 2, PON 2, aromatic esterase 2, arylesterase 2, paraoxonase nirs, serum aryldialkylphosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15165
  • Protein Name
    Serum paraoxonase/arylesterase 2
  • Scientific Description
    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PON2 using anti-PON2 antibody (A02027-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON2 antigen affinity purified polyclonal antibody (Catalog # A02027-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON2 at approximately 39 kDa. The expected band size for PON2 is at 39 kDa.
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