
POR antibody detects POR protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. POR antibody (GTX101099) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
POR antibody [N1N3]
GTX101099
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPOR
Overview
- SupplierGeneTex
- Product NamePOR antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.45 mg/ml
- ConjugateUnconjugated
- Gene ID5447
- Target namePOR
- Target descriptioncytochrome p450 oxidoreductase
- Target synonymsCPR, CYPOR, P450R, NADPH--cytochrome P450 reductase, NADPH--hemoprotein reductase, NADPH-dependent cytochrome P450 reductase, P450 (cytochrome) oxidoreductase
- HostRabbit
- IsotypeIgG
- Protein IDP16435
- Protein NameNADPH--cytochrome P450 reductase
- Scientific DescriptionThis gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Campelo D, Esteves F, Brito Palma B, et al. Probing the Role of the Hinge Segment of Cytochrome P450 Oxidoreductase in the Interaction with Cytochrome P450. Int J Mol Sci. 2018,19(12). doi: 10.3390/ijms19123914Read this paper
![Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N1N3] (GTX101099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N1N3] (GTX101099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101099/GTX101099_39925_20170323_WB_KO_watermark_w_23060100_913.webp)







