More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Pou4F1 Polyclonal Antibody

Pou4F1 Polyclonal Antibody

CAC11660
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPOU4F1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Biomatik
  • Product Name
    POU4F1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF; Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5457
  • Target name
    POU4F1
  • Target description
    POU class 4 homeobox 1
  • Target synonyms
    ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A, POU domain, class 4, transcription factor 1, brain-specific homeobox/POU domain protein 3A, homeobox/POU domain protein RDC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01851
  • Protein Name
    POU domain, class 4, transcription factor 1
  • Scientific Description
    The Pou4F1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Cusabio
POU4F1 AntibodyCSB-PA030134
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPOU4F1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-POU4F1 Antibody102-25805
ApplicationsWestern Blot
TargetPOU4F1
  • SizePrice
Figure 1. Western blot analysis of BRN3A using anti-BRN3A antibody (A05403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRN3A antigen affinity purified polyclonal antibody (Catalog # A05403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRN3A at approximately 45 kDa. The expected band size for BRN3A is at 43 kDa.
Product group Antibodies
Boster Bio
Anti-BRN3A/POU4F1 Antibody Picoband(r)A05403-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPOU4F1
  • SizePrice
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
BRN3A Polyclonal AntibodyBS-3669R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPOU4F1
  • SizePrice
ICC/IF analysis of PFA-fixed A549 cells using GTX04795 BRN3A antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100
Product group Antibodies
GeneTex
BRN3A antibodyGTX04795
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPOU4F1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
POU4F1 / BRN3A Antibody (280-360 aa, Internal)LS-C382312
ApplicationsWestern Blot, ELISA
TargetPOU4F1
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-POU4F1 AntibodyA96786
ApplicationsWestern Blot, ELISA
  • SizePrice