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ProductsBack/POU4F1 / BRN3A Antibody (280-360 aa, Internal)

POU4F1 / BRN3A Antibody (280-360 aa, Internal)

LS-C382312
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPOU4F1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POU4F1 / BRN3A Antibody (280-360 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5457
  • Target name
    POU4F1
  • Target description
    POU class 4 homeobox 1
  • Target synonyms
    ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A, POU domain, class 4, transcription factor 1, brain-specific homeobox/POU domain protein 3A, homeobox/POU domain protein RDC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BRN3A using anti-BRN3A antibody (A05403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRN3A antigen affinity purified polyclonal antibody (Catalog # A05403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRN3A at approximately 45 kDa. The expected band size for BRN3A is at 43 kDa.
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