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PPA1 antibody

GTX111031
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPPA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PPA1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5464
  • Target name
    PPA1
  • Target description
    inorganic pyrophosphatase 1
  • Target synonyms
    cytosolic inorganic pyrophosphatase; diphosphate phosphohydrolase; epididymis secretory sperm binding protein Li 66p; HEL-S-66p; inorganic diphosphatase 1; inorganic pyrophosphatase; IOPPP; PP; PP1; PPase; pyrophosphatase (inorganic) 1; pyrophosphatase 1; pyrophosphate phospho-hydrolase; SID6-8061
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15181
  • Protein Name
    Inorganic pyrophosphatase
  • Scientific Description
    The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPA1 using anti-PPA1 antibody (A07485-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPA1 antigen affinity purified polyclonal antibody (Catalog # A07485-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPA1 at approximately 33 kDa. The expected band size for PPA1 is at 33 kDa.
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