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PPEF / PPEF1 Antibody (aa1-201)

LS-C185492
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPPEF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PPEF / PPEF1 Antibody (aa1-201)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.12 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5475
  • Target name
    PPEF1
  • Target description
    protein phosphatase with EF-hand domain 1
  • Target synonyms
    PP7; PPEF; PPP7C; PPP7CA; protein phosphatase 7, catalytic subunit, alpha isozyme; protein phosphatase with EF calcium-binding domain; protein phosphatase, EF-hand calcium binding domain 1; protein phosphatase, serine/threonine type, with EF-hands; serine/threonine protein phosphatase 7; serine/threonine-protein phosphatase with EF-hands 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPEF1 using anti-PPEF1 antibody (A11074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPEF1 antigen affinity purified polyclonal antibody (Catalog # A11074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPEF1 at approximately 76 kDa. The expected band size for PPEF1 is at 76 kDa.
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