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Western blot All lanes: PPEF1 antibody at 1microg/ml Lane 1: U251 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Mouse gonadal tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 70, 73, 45, 69 kDa Observed band size: 76 kDa
Western blot All lanes: PPEF1 antibody at 1microg/ml Lane 1: U251 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Mouse gonadal tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 70, 73, 45, 69 kDa Observed band size: 76 kDa
Western blot All lanes: PPEF1 antibody at 1microg/ml Lane 1: U251 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Mouse gonadal tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 70, 73, 45, 69 kDa Observed band size: 76 kDa

PPEF1 Antibody

CSB-PA018435LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPPEF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPEF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5475
  • Target name
    PPEF1
  • Target description
    protein phosphatase with EF-hand domain 1
  • Target synonyms
    PP7, PPEF, PPP7C, PPP7CA, serine/threonine-protein phosphatase with EF-hands 1, protein phosphatase 7, catalytic subunit, alpha isozyme, protein phosphatase with EF calcium-binding domain, protein phosphatase, EF-hand calcium binding domain 1, protein phosphatase, serine/threonine type, with EF-hands, serine/threonine protein phosphatase 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14829
  • Protein Name
    Serine/threonine-protein phosphatase with EF-hands 1
  • Scientific Description
    May have a role in the recovery or adaptation response of photoreceptors. May have a role in development.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPEF1 using anti-PPEF1 antibody (A11074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPEF1 antigen affinity purified polyclonal antibody (Catalog # A11074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPEF1 at approximately 76 kDa. The expected band size for PPEF1 is at 76 kDa.
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