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Western Blot analysis of HuvEc cells using Cyclophilin F Polyclonal Antibody
Western Blot analysis of HuvEc cells using Cyclophilin F Polyclonal Antibody
Western Blot analysis of HuvEc cells using Cyclophilin F Polyclonal Antibody

PPIF Antibody

CSB-PA001907
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPPIF
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPIF Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10105
  • Target name
    PPIF
  • Target description
    peptidylprolyl isomerase F
  • Target synonyms
    cyclophilin 3; cyclophilin D; cyclophilin F; CYP3; CypD; Cyp-D; CyP-M; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; PPIase F; rotamase F
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30405
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase F, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
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Figure 1. Western blot analysis of Cyclophilin F using anti-Cyclophilin F antibody (M02803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin F antigen affinity purified monoclonal antibody (Catalog # M02803) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin F at approximately 18 kDa. The expected band size for Cyclophilin F is at 22 kDa.
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