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Western blot All lanes: PPM1Dantibody at 2.36ug/ml Lane 1: Rat gonadal tissue Lane 2: U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67, 48 kDa Observed band size: 67 kDa
Western blot All lanes: PPM1Dantibody at 2.36ug/ml Lane 1: Rat gonadal tissue Lane 2: U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67, 48 kDa Observed band size: 67 kDa
Western blot All lanes: PPM1Dantibody at 2.36ug/ml Lane 1: Rat gonadal tissue Lane 2: U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67, 48 kDa Observed band size: 67 kDa

PPM1D Antibody

CSB-PA018491ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetPPM1D
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPM1D Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8493
  • Target name
    PPM1D
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1D
  • Target synonyms
    IDDGIP, JDVS, PP2C-DELTA, WIP1, protein phosphatase 1D, protein phosphatase Wip1, wild-type p53-induced phosphatase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15297
  • Protein Name
    Protein phosphatase 1D
  • Scientific Description
    Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates Ser-15 of TP53 and Ser-345 of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629).
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IF analysis of PPM1D using anti-PPM1D antibody (A01576-1). PPM1D was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-PPM1D Antibody (A01576-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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