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Hela lysates probed with PPM1D Polyclonal Antibody, Unconjugated (bs-2447R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela lysates probed with PPM1D Polyclonal Antibody, Unconjugated (bs-2447R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela lysates probed with PPM1D Polyclonal Antibody, Unconjugated (bs-2447R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

PPM1D Polyclonal Antibody

BS-2447R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
TargetPPM1D
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Overview

  • Supplier
    Bioss
  • Product Name
    PPM1D Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8493
  • Target name
    PPM1D
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1D
  • Target synonyms
    IDDGIP, JDVS, PP2C-DELTA, WIP1, protein phosphatase 1D, protein phosphatase Wip1, wild-type p53-induced phosphatase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15297
  • Protein Name
    Protein phosphatase 1D
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang P, Cui J, Wen J, et al. Cisplatin induces HepG2 cell cycle arrest through targeting specific long noncoding RNAs and the p53 signaling pathway. Oncol Lett. 2016,12(6):4605-4612. doi: 10.3892/ol.2016.5288
    Read this paper

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Figure 2. IF analysis of PPM1D using anti-PPM1D antibody (A01576-1). PPM1D was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-PPM1D Antibody (A01576-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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