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PPM1E Rabbit mAb

A9374
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPPM1E
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Overview

  • Supplier
    ABclonal
  • Product Name
    PPM1E Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID22843
  • Target name
    PPM1E
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1E
  • Target synonyms
    CAMKPN, CaMKP-N, POPX1, PP2CH, caMKN, protein phosphatase 1E, ca(2+)/calmodulin-dependent protein kinase phosphatase N, caMKP-nucleus, nuclear calmodulin-dependent protein kinase phosphatase, partner of PIX 1, partner of PIX-alpha, partner of PIXA, protein phosphatase 1E (PP2C domain containing)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WY54
  • Protein Name
    Protein phosphatase 1E
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPM1E using anti-PPM1E antibody (A08794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1E antigen affinity purified polyclonal antibody (Catalog # A08794) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1E at approximately 84KD. The expected band size for PPM1E is at 84KD.
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