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Western Blot Positive WB detected in: Mouse liver tissue All lanes: PPM1F antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: PPM1F antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: PPM1F antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa

PPM1F Antibody

CSB-PA018493LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPPM1F
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPM1F Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9647
  • Target name
    PPM1F
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1F
  • Target synonyms
    CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2, protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, PP2C phosphatase, partner of PIX 2, protein fem-2 homolog, protein phosphatase 1F (PP2C domain containing)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49593
  • Protein Name
    Protein phosphatase 1F
  • Scientific Description
    Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPM1F using anti-PPM1F antibody (A07776-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1F antigen affinity purified polyclonal antibody (Catalog # A07776-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1F at approximately 54 kDa. The expected band size for PPM1F is at 50 kDa.
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