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Goat anti-PP2A / PPP2R1A Antibody

ORB18985
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetPPP2R1A
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-PP2A / PPP2R1A Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 60kDa band observed in Human Thymus and Lymph Node lysates (calculated MW of 65.3kDa according to NP_055040.2). Recommended concentration: 0.03-0.1 microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:4000, WB: 2-6 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5518
  • Target name
    PPP2R1A
  • Target description
    protein phosphatase 2 scaffold subunit Aalpha
  • Target synonyms
    HJS2, MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, medium tumor antigen-associated 61 KDA protein, protein phosphatase 2, regulatory subunit A, alpha, testicular secretory protein Li 1
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to PPP2R1A
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP2R1A using anti-PPP2R1A antibody (A02822-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R1A antigen affinity purified polyclonal antibody (Catalog # A02822-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R1A at approximately 65 kDa. The expected band size for PPP2R1A is at 65 kDa.
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