
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPP2R3A(GTX114655) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
PPP2R3A antibody [C2C3], C-term
GTX114655
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP2R3A
Overview
- SupplierGeneTex
- Product NamePPP2R3A antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5523
- Target namePPP2R3A
- Target descriptionprotein phosphatase 2 regulatory subunit B''alpha
- Target synonymsPPP2R3, PR130, PR72, serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, PR130/B''alpha1 subunit, PR72/B''alpha2 subuit, protein phosphatase 2, regulatory subunit B'', alpha, serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B
- HostRabbit
- IsotypeIgG
- Protein IDQ06190
- Protein NameSerine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha
- Scientific DescriptionProtein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B/PR61, and B/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B family. The B family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. Blue: Hoechst 33342 staining. PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114655/GTX114655_40590_IFA_w_23060518_274.webp)

![Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114655/GTX114655_40590_20210507_WB_M_R_w_23060518_946.webp)
![Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (GTX114655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114655/GTX114655_40590_20210507_WB_M_tissue_w_23060518_588.webp)






