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Anti-PPP3CC Antibody

CAB7714
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetPPP3CC
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-PPP3CC Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5533
  • Target name
    PPP3CC
  • Target description
    protein phosphatase 3 catalytic subunit gamma
  • Target synonyms
    CALNA3, CNA3, PP2Bgamma, serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform, CAM-PRP catalytic subunit, calcineurin, testis-specific catalytic subunit, protein phosphatase 3, catalytic subunit, gamma isozyme
  • Host
    Rabbit
  • Protein IDP48454
  • Protein Name
    Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform
  • Scientific Description
    PPP3CC Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PPP3CC using anti-PPP3CC antibody (A07461-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CC antigen affinity purified polyclonal antibody (Catalog # A07461-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CC at approximately 65 kDa. The expected band size for PPP3CC is at 58 kDa.
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