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Anti-PPP3R1 Antibody

ORB411631
Biorbyt
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP3R1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-PPP3R1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB: 1:500-2000, IHC-P: 1:50-200, IP: 1:50-100 IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5534
  • Target name
    PPP3R1
  • Target description
    protein phosphatase 3 regulatory subunit B, alpha
  • Target synonyms
    CALNB1, CNB, CNB1, calcineurin subunit B type 1, calcineurin B, type I (19kDa), protein phosphatase 2B regulatory subunit 1, protein phosphatase 2B regulatory subunit B alpha
  • Host
    Rabbit
  • Protein IDP63098
  • Protein Name
    Calcineurin subunit B type 1
  • Scientific Description
    Rabbit polyclonal antibody to PPP3R1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3R1 antigen affinity purified polyclonal antibody (Catalog # A06469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3R1 at approximately 15 kDa. The expected band size for PPP3R1 is at 15,19 kDa.
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