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ProductsBack/PR3 antibody [3B4]
WB analysis of PR3 (aa28-256)-hIgGFc transfected HEK293 cell lysate using GTX83362 PR3 antibody [3B4].
WB analysis of PR3 (aa28-256)-hIgGFc transfected HEK293 cell lysate using GTX83362 PR3 antibody [3B4].
WB analysis of PR3 (aa28-256)-hIgGFc transfected HEK293 cell lysate using GTX83362 PR3 antibody [3B4].

PR3 antibody [3B4]

GTX83362
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetPRTN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PR3 antibody [3B4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B4
  • Conjugate
    Unconjugated
  • Gene ID5657
  • Target name
    PRTN3
  • Target description
    proteinase 3
  • Target synonyms
    ACPA; AGP7; azurophil granule protein 7; CANCA; C-ANCA; C-ANCA antigen; leukocyte proteinase 3; MBN; MBT; myeloblastin; neutrophil proteinase 4; NP4; NP-4; P29; PR3; PR-3; serine proteinase, neutrophil; wegener autoantigen; Wegener granulomatosis autoantigen
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP24158
  • Protein Name
    Myeloblastin
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody (Catalog # A03863-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRTN3 at approximately 32 kDa. The expected band size for PRTN3 is at 28,32 kDa.
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