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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA102179(PRC1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA102179(PRC1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA102179(PRC1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

PRC1 Antibody

CSB-PA102179
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPRC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9055
  • Target name
    PRC1
  • Target description
    protein regulator of cytokinesis 1
  • Target synonyms
    ASE1, MAP65, protein regulator of cytokinesis 1, anaphase spindle elongation 1 homolog, protein regulating cytokinesis 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43663
  • Protein Name
    Protein regulator of cytokinesis 1
  • Scientific Description
    This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: SGC Whole Cell Lysate, Lane 3: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (Catalog # PB9814) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRC1 at approximately 75 kDa, 80 kDa. The expected band size for PRC1 is at 75 kDa, 80 kDa.
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