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WB analysis of rat testis tissue lysate using GTX54571 PRDM14 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of rat testis tissue lysate using GTX54571 PRDM14 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of rat testis tissue lysate using GTX54571 PRDM14 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25μg per lane

PRDM14 antibody

GTX54571
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRDM14
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRDM14 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. ChIP assay: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63978
  • Target name
    PRDM14
  • Target description
    PR/SET domain 14
  • Target synonyms
    PFM11, PR domain zinc finger protein 14, PR domain 14, PR domain-containing 14, PR domain-containing protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZV8
  • Protein Name
    PR domain zinc finger protein 14
  • Scientific Description
    This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PRDM14 using anti-PRDM14 antibody (A03870-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM14 antigen affinity purified polyclonal antibody (Catalog # A03870-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM14 at approximately 64KD. The expected band size for PRDM14 is at 64KD.
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