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Western blot analysis of extracts of various cell lines using PRDM14 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PRDM14 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PRDM14 Polyclonal Antibody at 1:1000 dilution.

PRDM14 Polyclonal Antibody

E-AB-64775
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PRDM14 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRDM14 using anti-PRDM14 antibody (A03870-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM14 antigen affinity purified polyclonal antibody (Catalog # A03870-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM14 at approximately 64KD. The expected band size for PRDM14 is at 64KD.
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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA246357(PRDM14 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
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