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Western Blot analysis of HeLa cells using PRX III Polyclonal Antibody
Western Blot analysis of HeLa cells using PRX III Polyclonal Antibody
Western Blot analysis of HeLa cells using PRX III Polyclonal Antibody

PRDX3 Antibody

CSB-PA003861
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRDX3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRDX3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10935
  • Target name
    PRDX3
  • Target description
    peroxiredoxin 3
  • Target synonyms
    AOP-1, AOP1, HBC189, MER5, PPPCD, PRO1748, SCAR32, SP-22, prx-III, thioredoxin-dependent peroxide reductase, mitochondrial, antioxidant protein 1, epididymis secretory sperm binding protein, peroxiredoxin III, protein MER5 homolog, thioredoxin-dependent peroxiredoxin 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30048
  • Protein Name
    Thioredoxin-dependent peroxide reductase, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti PRDX3/peroxiredoxin 3Polyclonal Antibody, Unconjugated (bs-1874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.\n
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Figure 1. Western blot analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Mouse Brain Tissue Lysate, Lane 3: Rat Skeletal Muscle Tissue Lysate, Lane 4: Mouse Skeletal Muscle Tissue Lysate, Lane 5: U20S Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: SMMC Whole Cell Lysate, Lane 8: RH35 Whole Cell Lysate, Lane 9: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9349) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 3 at approximately 25KD. The expected band size for Peroxiredoxin 3 is at 25KD.
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