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ProductsBack/PRDX3 / Peroxiredoxin 3 Antibody

PRDX3 / Peroxiredoxin 3 Antibody

LS-C769842
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPRDX3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRDX3 / Peroxiredoxin 3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10935
  • Target name
    PRDX3
  • Target description
    peroxiredoxin 3
  • Target synonyms
    AOP-1, AOP1, HBC189, MER5, PPPCD, PRO1748, SCAR32, SP-22, prx-III, thioredoxin-dependent peroxide reductase, mitochondrial, antioxidant protein 1, epididymis secretory sperm binding protein, peroxiredoxin III, protein MER5 homolog, thioredoxin-dependent peroxiredoxin 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti PRDX3/peroxiredoxin 3Polyclonal Antibody, Unconjugated (bs-1874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 28kD. Observed band size: 28kD.\n
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Figure 1. Western blot analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Mouse Brain Tissue Lysate, Lane 3: Rat Skeletal Muscle Tissue Lysate, Lane 4: Mouse Skeletal Muscle Tissue Lysate, Lane 5: U20S Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: SMMC Whole Cell Lysate, Lane 8: RH35 Whole Cell Lysate, Lane 9: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9349) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 3 at approximately 25KD. The expected band size for Peroxiredoxin 3 is at 25KD.
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