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Presenilin 1 antibody

20-PG71
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    Presenilin 1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PSEN1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 43, 22, 47, 49 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of Presenilin 1/PS-1/PSEN1 using anti-Presenilin 1/PS-1/PSEN1 antibody (A00138-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 1/PS-1/PSEN1 antigen affinity purified polyclonal antibody (Catalog # A00138-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Presenilin 1/PS-1/PSEN1 at approximately 53 kDa. The expected band size for Presenilin 1/PS-1/PSEN1 is at 53 kDa.
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