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Presenilin 2 Antibody (YA1605, Rabbit)

HY-P81860
MedChem Express
TargetPSEN2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Presenilin 2 Antibody (YA1605, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5664
  • Target name
    PSEN2
  • Target description
    presenilin 2
  • Target synonyms
    AD3L, AD4, CMD1V, PS2, STM2, presenilin-2, AD3LP, AD5, Alzheimer disease 3-like, Alzheimer disease 4, E5-1, PS-2, STM-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Presenilin 2 Antibody (YA1605) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1605), targeting Presenilin 2, with a predicted molecular weight of 50 kDa (observed band size: 23 kDa). Presenilin 2 Antibody (YA1605) can be used for WB, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (A00800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # A00800) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD.
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