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ProductsBack/PREX1 / P-REX1 Antibody (Preservative Free)

PREX1 / P-REX1 Antibody (Preservative Free)

LS-C815000
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPREX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PREX1 / P-REX1 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57580
  • Target name
    PREX1
  • Target description
    phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1
  • Target synonyms
    phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; PIP3 dependent Rac exchange factor 1; P-REX1; ptdIns(3,4,5)-dependent Rac exchanger 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PREX1 using anti-PREX1 antibody (A03098-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX1 antigen affinity purified polyclonal antibody (Catalog # A03098-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX1 at approximately 186 kDa. The expected band size for PREX1 is at 186 kDa.
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