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PRF1 Antibody (OAED00295)

OAED00295
Aviva Systems Biology
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRF1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PRF1 Antibody (OAED00295)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CB5.4
  • Concentration
    1 mg/ml
  • Format
    Liquid. In PBS containing 0.02% sodium azide
  • Gene ID5551
  • Target name
    PRF1
  • Target description
    perforin 1
  • Target synonyms
    cytolysin; HPLH2; lymphocyte pore-forming protein; P1; perforin 1 (pore forming protein); perforin-1; PFP
  • Host
    Rat
  • Isotype
    IgG2a
  • Source
    Purified from mouse ascites
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Perforin using anti-Perforin antibody (PB9155). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Perforin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perforin antigen affinity purified polyclonal antibody (Catalog # PB9155) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perforin at approximately 40 kDa. The expected band size for Perforin is at 40 kDa.
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