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IHC image of CSB-RA051594A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.
Product group Antibodies
Cusabio
Phospho-MAPT (S199) Recombinant Monoclonal AntibodyCSB-RA051594A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAPT
Western Blot Positive WB detected in: U251 whole cell lysate All lanes: EAAT2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Product group Antibodies
Cusabio
SLC1A2 Recombinant Monoclonal AntibodyCSB-RA051685A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetSLC1A2
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate All lanes: EBI3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140 kDa Observed band size: 140 kDa
Product group Antibodies
Cusabio
PKN2 Recombinant Monoclonal AntibodyCSB-RA056240A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPKN2
IHC image of CSB-RA058699A0HU diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.16% DAB.
Product group Antibodies
Cusabio
TNNT2 Recombinant Monoclonal AntibodyCSB-RA058699A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTNNT2
Overlay Peak curve showing RAJI cells stained with CSB-RA062241A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
BECN1 Recombinant Monoclonal AntibodyCSB-RA062241A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetBECN1
Immunofluorescence staining of MCF-7 with CSB-RA067358A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 493-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
USP22 Recombinant Monoclonal AntibodyCSB-RA067358A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetUSP22
Immunofluorescence staining of A549 with CSB-RA069971A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 520-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
PITX3 Recombinant Monoclonal AntibodyCSB-RA069971A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetPITX3
IHC image of CSB-RA071064A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.
Product group Antibodies
Cusabio
HSD3B1 Recombinant Monoclonal AntibodyCSB-RA071064A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHSD3B1
IHC image of CSB-RA072684A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.32% DAB.
Product group Antibodies
Cusabio
HIST1H1C Recombinant Monoclonal AntibodyCSB-RA072684A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH1-2
IHC image of CSB-RA076873A0HU diluted at 1:50 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.
Product group Antibodies
Cusabio
ATP2A2 Recombinant Monoclonal AntibodyCSB-RA076873A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetATP2A2
Western Blot Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, All lanes: CREB antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Product group Antibodies
Cusabio
CREB1 Recombinant Monoclonal AntibodyCSB-RA081853A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCREB1
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA082445A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
Phospho-MAPT (S202) Recombinant Monoclonal AntibodyCSB-RA082445A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetMAPT
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