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Western Blot Positive WB detected in: A549 whole cell lysate, U251 whole cell lysate,MCF7 whole cell lysate,HELA whole cell lysate,PC-3 whole cell lysate All lanes: Glucose 6 phosphate isomerase antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa
Product group Antibodies
Cusabio
GPI Recombinant Monoclonal AntibodyCSB-RA130853A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGPI
IHC image of CSB-RA131953A0HU diluted at 1:33 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.73% DAB.
Product group Antibodies
Cusabio
BCL6 Recombinant Monoclonal AntibodyCSB-RA131953A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBCL6
IHC image of CSB-RA148519A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.
Product group Antibodies
Cusabio
Formyl-HIST1H3A (K122) Recombinant Monoclonal AntibodyCSB-RA148519A0HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetH3C1
IHC image of CSB-RA150369A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.58% DAB.
Product group Antibodies
Cusabio
DYNLL1 Recombinant Monoclonal AntibodyCSB-RA150369A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDYNLL1
Overlay Peak curve showing HepG2 cells stained with CSB-RA153094A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
DEFB1 Recombinant Monoclonal AntibodyCSB-RA153094A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetDEFB1
IHC image of CSB-RA156336A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.
Product group Antibodies
Cusabio
LGR6 Recombinant Monoclonal AntibodyCSB-RA156336A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetLGR6
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
EGFR Recombinant Monoclonal AntibodyCSB-RA159341MA1HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetEGFR
IHC image of CSB-RA175460A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.
Product group Antibodies
Cusabio
ATP7B Recombinant Monoclonal AntibodyCSB-RA175460A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetATP7B
IHC image of CSB-RA176322A0HU diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.61% DAB.
Product group Antibodies
Cusabio
KRT20 Recombinant Monoclonal AntibodyCSB-RA176322A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKRT20
IHC image of CSB-RA18201:50A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.
Product group Antibodies
Cusabio
WNK1 Recombinant Monoclonal AntibodyCSB-RA182028A0HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetWNK1
Overlay Peak curve showing Hela cells stained with CSB-RA182340A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
NUMA1 Recombinant Monoclonal AntibodyCSB-RA182340A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetNUMA1
Western Blot Positive WB detected in: PC-3 whole cell lysate,Hela whole cell lysate All lanes: Placental alkaline phosphatase (PLAP) antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa
Product group Antibodies
Cusabio
ALPP Recombinant Monoclonal AntibodyCSB-RA183247A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetALPP
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