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Simple Western™ analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody #TA500381. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50  dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Monkey
TargetTRPM4
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9. Positive lysates LY402335 (100ug) and LC402335 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Human, Monkey
TargetSIGLEC9
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetPLK1
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetCHEK2
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetCHEK2
  • SizePrice
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human TrkB  plasmid (RC205129, hTrkB), mouse TrkB plasmid (MR226130, mTrkB) using anti-TrkB antibody TA500386 (1:20000@1mg/ml). Figure B, Western blot analysis of  the same samples as figure A with anti-DDK antibody (TA180144, 1:20000@1mg/ml)
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNTRK2
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetPLK1
  • SizePrice
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PLK1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPLK1
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetCHEK2
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetCHEK2
  • SizePrice
Equivalent amounts of  cell lysates (30 ug per lane) of  wild-type HepG2 cells(WT) and OXSR1-Knockdown HepG2 cells(KD)  were separated by SDS-PAGE and immunoblotted with anti-OXSR1 monoclonal antibody TA500392(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetOXSR1
  • SizePrice
Anti-PLK1 mouse monoclonal antibody (TA500393) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1 (RC201795) (1:100).
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPLK1
  • SizePrice

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