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Detection of endogenous PKB: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab 11A11 (1 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: Hela; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT; lane 8: Neuro 2A; lane 9: 3T3
Product group Antibodies
OriGene
AKT1 non pSer473 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11A11]AM00107PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
AKT1 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5G12]AM00108PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Rat
TargetAKT1
  • SizePrice
Detection of endogenous akt2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PKB-8B7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D
Product group Antibodies
OriGene
AKT2 (specific) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 8B7]AM00109PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAKT2
  • SizePrice
Product group Antibodies
OriGene
AKT1 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5C10]AM00110BT-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Detection of endogenous PKB Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab PKB-5C10 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). lane 1: SW480; lane 2: SW620; lane 3: HT29; lane 4: MCF-7; lane 5: MDA-MB 231; lane 6: T47D
Product group Antibodies
OriGene
AKT1 (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5C10]AM00110PU-N
ApplicationsWestern Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
PKG Substrate pSer (activated) Mouse Monoclonal Antibody [Clone ID: 7H1]AM00111PU-N
ApplicationsELISA
  • SizePrice
Phosphoserine Detection. Phosphoprotein Positive Control was probed with: Lane 1: mab 1C8 (IgM), 1 g/ml Lane 2: mab 4A3 (IgM), 1 g/ml Lane 3: mab 4A9 (IgM), 1 g/ml Lane 4: mab 4H4 (IgM), 1 g/ml Lane 5: mab 7F12 (IgG), 1 g/ml Lane 6: mab 16B4 (IgM), 1 g/ml
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1C8]AM00112PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Figure 1. Phosphoserine Detection: Phosphoprotein Positive Control was probed with lane 1: mab 1C8 (IgM), 1 g/ml lane 2: mab 4A3 (IgM), 1 g/ml lane 3: mab 4A9 (IgM), 1 g/ml lane 4: mab 4H4 (IgM), 1 g/ml lane 5: mab 7F12 (IgG), 1 g/ml lane 6: mab 16B4 (IgM)
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4A3]AM00113PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Phosphoserine Detection. Phosphoprotein Positive Control was probed with: Lane 1: mab 1C8 (IgM), 1 g/ml Lane 2: mab 4A3 (IgM), 1 g/ml Lane 3: mab 4A9 (IgM), 1 g/ml Lane 4: mab 4H4 (IgM), 1 g/ml Lane 5: mab 7F12 (IgG), 1 g/ml Lane 6: mab 16B4 (IgM), 1 g/ml
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4A9]AM00114PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Phosphoserine Detection: Phosphoprotein Positive Control was probed with Lane 1: mab 1C8 (IgM), 1 g/ml Lane 2: mab 4A3 (IgM), 1 g/ml Lane 3: mab 4A9 (IgM), 1 g/ml Lane 4: mab 4H4 (IgM), 1 g/ml Lane 5: mab 7F12 (IgG), 1 g/ml Lane 6: mab 16B4 (IgM), 1 g/ml
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4H4]AM00115PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Phosphoserine Detection: Phosphoprotein Positive Control was probed with: Lane 1: mab 1C8 (IgM), 1 g/ml. Lane 2: mab 4A3 (IgM), 1 g/ml. lane 3: mab 4A9 (IgM), 1 g/ml. Lane 4: mab 4H4 (IgM), 1 g/ml. Lane 5: mab 7F12 (IgG), 1 g/ml. Lane 6: mab 16B4 (IgM), 1
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]AM00116PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
Phosphoserine Detection: Phosphoprotein Positive Control was probed with: Lane 1: mab 1C8 (IgM), 1 g/ml Lane 2: mab 4A3 (IgM), 1 g/ml Lane 3: mab 4A9 (IgM), 1 g/ml Lane 4: mab 4H4 (IgM), 1 g/ml Lane 5: mab 7F12 (IgG), 1 g/ml Lane 6: mab 16B4 (IgM), 1 g/ml
Product group Antibodies
OriGene
Phosphoserine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16B4]AM00117PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
  • SizePrice
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