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IHC image of CSB-RA004928MA1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD37
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: CD3D antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/50000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCD3D
Overlay Peak curve showing JK cells stained with CSB-RA004931MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 006 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCD3E
Immunofluorescence staining of A549 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetCD40
Overlay Peak curve showing Hela cells stained with CSB-RA004938MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCD44
The Binding Activity of Human CD69 with Anti-CD69 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD69 (CSB-MP004952HU) at 2 microg/mL can bind Anti-CD69 recombinant antibody , the EC50 is 23.17-26.04 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCD69
The Binding Activity of Human CD70 with Anti-CD70 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD70 (CSB-MP004954HU1) at 2 microg/mL can bind Anti-CD70 recombinant antibody, the EC50 is 1.642-2.005 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCD70
The Binding Activity of Human CD81 with Anti-CD81 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD81(CSB-MP004960HUd7) at 2 microg/mL can bind Anti-CD81 recombinant antibody. the EC50 is 4.166-5.578 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCD81
Western Blot Positive WB detected in: Mouse Liver tissue lysate All lanes: CDH1 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 80-135 kDa Observed band size: 135 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCDH1
The Binding Activity of Human CDH1 with Anti-CDH1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CDH1(CSB-MP005034HU1) at 2 microg/mL can bind Anti-CDH1 recombinant antibody.The EC50 is 5.151-6.358 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCDH1
The Binding Activity of Human CDH6 with Anti-CDH6 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CDH6 (CSB-MP005055HU1) at 2 microg/mL can bind Anti-CDH6 recombinant antibody. The EC50 is 2.421-2.802 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCDH6
The Binding Activity of Human CEACAM5 with Anti-CEACAM5 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 (CSB-MP005165HU) at 2microg/mL can bind Anti-CEACAM5 recombinant antibody, the EC50 is 0.8955-1.719 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCEACAM5