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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: Caspase 3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCASP3
IHC image of CSB-RA003657MA1HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetC1S
Overlay Peak curve showing Hela cells stained with CSB-RA003995MA1HU (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 021 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetC5
Western Blot Positive WB detected in: 293 whole cell lysate All lanes: CALR antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCALR
IHC image of CSB-RA004783MA1HU diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCCL2
The Binding Activity of Human CCR5 with Anti-CCR5 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR5 (CSB-MP004844HU) at 10 microg/mL can bind Anti-CCR5 recombinant antibody. The EC50 is 1.099-1.287 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCCR5
The Binding Activity of Human CCR6 with Anti-CCR6 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR6 (CSB-MP004845HU) at 10 microg/mL can bind Anti-CCR6 recombinant antibody. The EC50 is 44.79-56.10 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCCR6
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCCR6
The Binding Activity of CCR9 with Anti-CCR9 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR9 at 10 microg/mL can bind Anti-CCR9 recombinant antibody (CSB-RA004848MA1HU). The EC50 is 31.67-36.83 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetCCR9
IHC image of CSB-RA004879MA1HU diluted at 1 : 100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD14
Western Blot Positive WB detected in: Raji whole cell lysate,JK whole cell lysate All lanes: CD19 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCD19
Immunofluorescence staining of Hela cell with CSB-RA004888MA2HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetCD19