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ProductsBack/PRKAR1 Recombinant Antibody, AbBy Fluor-555 Conjugated

PRKAR1 Recombinant Antibody, AbBy Fluor-555 Conjugated

BSM-61856R-BF555
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPRKAR1A
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Overview

  • Supplier
    Bioss
  • Product Name
    PRKAR1 Recombinant Antibody, AbBy Fluor-555 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID5573
  • Target name
    PRKAR1A
  • Target description
    protein kinase cAMP-dependent type I regulatory subunit alpha
  • Target synonyms
    ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, Prkar1alpha, TSE1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Carney complex type 1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, epididymis secretory sperm binding protein, protein kinase A type 1a regulatory subunit, protein kinase, cAMP-dependent, regulatory subunit type I alpha, protein kinase, cAMP-dependent, regulatory, type I, alpha, tissue-specific extinguisher 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10644
  • Protein Name
    cAMP-dependent protein kinase type I-alpha regulatory subunit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR1A antigen affinity purified polyclonal antibody (Catalog # A00699-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAR1A at approximately 48 kDa. The expected band size for PRKAR1A is at 43KD.
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