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Western Blot analysis of 293 cells using PKA Ialpha reg Polyclonal Antibody
Western Blot analysis of 293 cells using PKA Ialpha reg Polyclonal Antibody
Western Blot analysis of 293 cells using PKA Ialpha reg Polyclonal Antibody

PRKAR1A Antibody

CSB-PA003796
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKAR1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRKAR1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5573
  • Target name
    PRKAR1A
  • Target description
    protein kinase cAMP-dependent type I regulatory subunit alpha
  • Target synonyms
    ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, Prkar1alpha, TSE1, cAMP-dependent protein kinase type I-alpha regulatory subunit, Carney complex type 1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, epididymis secretory sperm binding protein, protein kinase A type 1a regulatory subunit, protein kinase, cAMP-dependent, regulatory subunit type I alpha, protein kinase, cAMP-dependent, regulatory, type I, alpha, tissue-specific extinguisher 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10644
  • Protein Name
    cAMP-dependent protein kinase type I-alpha regulatory subunit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR1A antigen affinity purified polyclonal antibody (Catalog # A00699-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAR1A at approximately 48 kDa. The expected band size for PRKAR1A is at 43KD.
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