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ProductsBack/PRKCB / PKC-Beta Antibody

PRKCB / PKC-Beta Antibody

LS-C760925
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPRKCB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRKCB / PKC-Beta Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5579
  • Target name
    PRKCB
  • Target description
    protein kinase C beta
  • Target synonyms
    PKC-beta, PKCB, PKCI(2), PKCbeta, PRKCB1, PRKCB2, protein kinase C beta type, PKC-B, protein kinase C, beta 1 polypeptide
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRKCB using anti-PRKCB antibody (A01940). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCB antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCB at approximately 77 kDa. The expected band size for PRKCB is at 77 kDa.
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