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PRKRA Antibody (YA1522, Rabbit)

HY-P81777
MedChem Express
TargetPRKRA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PRKRA Antibody (YA1522, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8575
  • Target name
    PRKRA
  • Target description
    protein activator of interferon induced protein kinase EIF2AK2
  • Target synonyms
    DYT16, HSD14, PACT, RAX, interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, protein activator of the interferon-induced protein kinase, protein kinase, interferon-inducible double-stranded RNA-dependent activator
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PRKRA Antibody (YA1522) is a non-conjugated IgG antibody, targeting PRKRA, with a predicted molecular weight of 34 kDa (observed band size: 34 kDa). PRKRA Antibody (YA1522) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRKRA using anti-PRKRA antibody (A02744-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKRA antigen affinity purified polyclonal antibody (Catalog # A02744-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKRA at approximately 32 kDa. The expected band size for PRKRA is at 32 kDa.
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