ChIP analysis of HCT116 cells using GTX47433 PRMT6 antibody. Use 0.5microg antibody GTX47433 PRMT6 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
ChIP analysis of HCT116 cells using GTX47433 PRMT6 antibody. Use 0.5microg antibody GTX47433 PRMT6 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
PRMT6 antibody, Internal
GTX47433
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetPRMT6
Overview
- SupplierGeneTex
- Product NamePRMT6 antibody, Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- ConjugateUnconjugated
- Gene ID55170
- Target namePRMT6
- Target descriptionprotein arginine methyltransferase 6
- Target synonymsheterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; HRMT1L6; protein arginine N-methyltransferase 6
- HostRabbit
- IsotypeIgG
- Protein IDQ96LA8
- Protein NameProtein arginine N-methyltransferase 6
- Scientific DescriptionThe protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203